卵泡期和黄体期山羊垂体miRNAs表达谱及差异分析

doi:10.11843/j.issn.0366-6964.2016.11.009

Abstract

Abstract:

In this study, we used Solexa sequencing to screen and analyze the differentially expressed microRNAs (miRNAs) of Anhuai goat pituitary tissues in the follicular phase (Fols) and luteal phase (Luts), and explore the role of pituitary at post-transcriptional level of Fols to Luts transition occurred in goats. In total, 11 319 069 and 11 432 846 raw reads were obtained from the ovaries of Anhuai goats in Fols and Luts, respectively, after eliminating impurity, 11 162 888 and 11 016 138 clean reads. 147 known miRNAs were co-expressed in the two different periods phases, 148 and 150 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 27 novel miRNAs were co-expressed in the two phases, 52 and 95 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNAs in the two phases, and miR-159 was the highest expressed significantly different novel miRNAs in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. In the KEGG pathway analysis, ko00511, ko00052, ko01100, ko00030 and ko00520 may be related to the synthesis of sex hormones. The study succeeded to construct the expression library of miRNAs which were abundant and differentially expressed in Anhuai goat pituitary tissues. The results will help to further understand the role of miRNAs which regulates secretion of hormone participate in goat pituitary. And some identified miRNAs will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries.

CLC Number:

S827.2

LING Ying-hui,ZHU Long,SUI Meng-hua,ZHENG Qi,WU Hao,ZHANG Yun-hai,ZHANG Xiao-rong. Comparative Profiling of Differentially Expressed microRNAs between the Follicular and Luteal Phases Pituitary of Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2218-2227.

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Comparative Profiling of Differentially Expressed microRNAs between the Follicular and Luteal Phases Pituitary of Goats

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